Characterization of Aeromonas hydrophila through RAPD-PCR and SDS-PAGE Analysis
نویسندگان
چکیده
منابع مشابه
Characterization of Aeromonas hydrophila through RAPD-PCR and SDS-PAGE Analysis
Aeromonas hydrophila isolated from fish (Labeo rohita), pond water, river water, raw meat of chicken and mutton and raw cow milk were characterized through Random Amplified Polymorphic DNA (RAPD) analysis and Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS PAGE) of cellular proteins. RAPD-PCR amplification of bacterial DNA was done by using ten random primers (OPA-01 to 10) and ...
متن کاملDetection and characterization of the hemolysin genes in Aeromonas hydrophila and Aeromonas sobria by multiplex PCR.
A multiplex PCR assay was designed to amplify the Aeromonas hydrophila and A. veronii bv. sobria hemolysin and aerolysin genes. The assay was evaluated by using 121 clinical isolates and 7 reference strains of Aeromonas spp., and these were divided into five genotypes on the basis of the results of the multiplex PCR. The five genotypes were characterized as type 1 for those carrying the ahh1 ge...
متن کاملSDS-PAGE heat-shock protein profiles of environmental Aeromonas strains.
Aeromonas microorganisms normally grow at temperatures between 5 degrees C and 45 degrees C and therefore should have high thermotolerance. Thus it was of interest to find out whether A. hydrophila, A. caviae and A. veronii biovar sobria serovars respond to abrupt temperature changes with a heat shock-like response. To this end the present study was undertaken to determine whether Aeromonas spe...
متن کاملCharacterization of lactoferrin binding by Aeromonas hydrophila.
Various lactoferrin preparations (iron-saturated and iron-depleted human milk lactoferrins and bovine milk and colostrum lactoferrins) were bound by Aeromonas hydrophila. Binding was (i) reversible (65% of bound lactoferrin was displaced by unlabeled lactoferrin), (ii) specific (lactoferrin but not other iron-containing glycoproteins such as ferritin, transferrin, hemoglobin, and myoglobin inhi...
متن کاملDifferentiating Panax ginseng and Panax quinquefolius through SDS-PAGE Analysis
Ginseng is a traditional Chinese herb commonly used in the formulation of tonics. There are 2 main varieties: Oriental (Panax ginseng) and American ginsengs (Panax quinquefolius). Traditional means of authenticating ginseng have become less reliable since they are being processed into various formulations such as tablets, capsules, powder and tea. These products are sometimes also adulterated. ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Open Journal of Medical Microbiology
سال: 2012
ISSN: 2165-3372,2165-3380
DOI: 10.4236/ojmm.2012.22005